Preparation of Nucleosome Core Particles Complexed with DNA Repair Factors for Cryo-Electron Microscopy Structural Determination

Scientific Article | Ce protocole décrit en détail la préparation des complexes nucléosomiques à l’aide de deux méthodes de préparation des échantillons
DNA repair in the context of chromatin is poorly understood. Biochemical studies using nucleosome core particles, the fundamental repeating unit of chromatin, show most DNA repair enzymes remove DNA damage at reduced rates as compared to free DNA.

Preparation of Nucleosome Core Particles Complexed with DNA Repair

The cryo-EM resolution revolution and transcription complexes

Cryo-EM structure of the human Sirtuin 6–nucleosome complex

Cryo-EM structure of the nucleosome containing the ALB1 enhancer

Phase-plate cryo-EM analysis of Nucleosome Core Particles (NCPs

Participation of Histones in DNA Damage and Repair within

Molecular organization of the early stages of nucleosome phase

The cryo‐EM structure of the CENP‐A nucleosome in complex with

Cryo-EM structure of the SMARCAD1-nucleosome complex, revealing a

Cryo-electron microscopy structure of the H3-H4 octasome without