Electrophoretic mobility shift assay (EMSA) showing the association

Download scientific diagram | | Electrophoretic mobility shift assay (EMSA) showing the association of SlHY5 with promoters of SGA biosynthetic genes SlGAME1, SlGAME4, and SlGAME17. (A) SDS-PAGE gel stained with Coomassie blue showing affinity purification of the recombinant SlHY5 protein used for the EMSA. (B) SlHY5 binds directly to the G-box (CACGTG) element of the promoters of SlGAME1, SlGAME4, and SlGAME17. Biotin-labeled DNA probes for the promoters or mutated probes were incubated with GST-HY5 protein and the DNA-protein complexes were separated on 6% native polyacrylamide gels. + or ++ indicate increasing amounts of unlabeled probes as the competitor. from publication: Manipulation of Light Signal Transduction Factors as a Means of Modifying Steroidal Glycoalkaloids Accumulation in Tomato Leaves | Steroidal glycoalkaloids (SGAs) are cholesterol-derived specialized metabolites produced by Solanaceous plant species. They contribute to pathogen defense but are considered as anti-nutritional compounds and toxic to humans. Although the genes involved in the SGA biosynthetic | Tomato, Lycopersicon esculentum and Light Signal Transduction | ResearchGate, the professional network for scientists.